Reaction tray operation

When initialized, the tray makes a maximum of two, counterclockwise turns then comes to a stop when RRV cuvette 1 is in position for the first reagent.

After you start a run, the reaction tray moves RRV cuvette 1 counterclockwise to the reagent 1 dispense position. Then the following cycle begins:

  1. R1 is dispensed into the RRV cuvette.

  2. The reaction tray moves three cycles counterclockwise, turning past 49 cuvettes per cycle and moving RRV cuvette 1 to the sample dispense position.

Meanwhile, an empty cuvette moves to the R1 dispense position, beginning the analysis cycle for the next sample.

  1. Sample is dispensed into the cuvette.

  2. During the next 12 seconds, the cuvette moves another 6 cycles to the mixer 1 (MIXR1) position.

  3. MIXR1 mixes the reagent and sample.

As the analysis progresses, the spectrophotometer measures the absorbance of the liquid in each of the cuvettes that pass it (across all 14 wavelengths).

  1. R1 and sample are dispensed into subsequent cuvettes and mixed, until all tests have completed and data has been collected.

When required by assay conditions, the RRV stops for dispensation and mixing of R2.

You can view measurement data in the Reaction Monitor window.

  1. After the required measurements complete, cuvettes used for assay are washed and the cell blank absorbance is checked.

During each reaction tray movement, unused cuvettes are washed with cell conditioner. Then a cell blank measurement is performed. You can view cell blank results in the Reaction Monitor window.

If an assay is not running, you can operate the unit manually from the Manual Operation window.