For a given method, three sets of reanalysis conditions are available at the Analytical Parameters (Chemistry) window.
One set allows a repeat analysis with unchanged conditions. Two sets are used to reanalyze a sample under conditions where the dilution ratio of sample to saline is changed. For all methods reanalysis is always performed using the pre-diluted sample that is maintained in the dilution tray (DTT).
Samples that has an analyte concentration either below or above the detection range of a method may require a reanalysis to check a result or obtain a result.
The three reanalysis conditions are M, U, and D.
|
M |
Reanalysis using unchanged conditions |
|
U or D |
Reanalysis conditions that may require further dilutions of the pre-diluted DTT sample or the use of an increased or decreased Serum reac. Smp. Vol. |
Normal analysis requests use the M conditions. Although U and D reanalysis is automatic, you can request either condition directly from the Order Entry window.
To implement the automatic reanalysis, enter the following information for each method in the Analytical Parameters (Chemistry) window:
· Absorbance limit settings (u) and (d)
· Reanalysis conditions (u) and (d) for samples exceeding the absorbance limits
· The appropriate instructions in the Reanalysis Conditions Set
Before running reanalysis, select DTT retest or STT retest in the System operation basic spec area of the System Specification Settings window.
For all Endpoint methods (EPA) the absorbance limits are entered as Re. Absorb (u) and (d).
EPA methods measuring absorbance increases use the Re.absorb (u) limit to detect results above the assay range and Re.absorb (d) limit for results below the range. Samples exceeding these limits are reanalyzed using the (u) and (d) conditions respectively.
EPA methods measuring absorbance decreases use the Re.absorb (d) limit to detect results above the assay range and Re.absorb (u) limit for results below the range. Samples exceeding these limits are reanalyzed using the (d) and (u) conditions respectively.
For all Rate reaction methods (RRA) the absorbance limits are entered as Sample (u) and Sample (d).
RRA methods measuring absorbance increases use Sample (u) limit to detect results above the assay range and Sample (d) limits for results below the range. Samples exceeding these limits are reanalyzed using the (u) and (d) conditions respectively.
RRA methods measuring absorbance decreases use the Sample (d) limit to detect results above the assay range and Sample (u) limit for results below the range. Samples exceeding these limits are reanalyzed using the (d) and (u) conditions respectively.
RRA methods also require input of Blank (u) and (d) absorbances which are used as a reagent integrity test and do not influence reanalysis. These absorbance values are the allowable reagent tolerances and are utilized during reagent blank procedures and if a method has a check D.P.I.
Select here for a complete listing of the method parameters including those used for reanalysis.