Example C: Calibrating Direct Bilirubin using Total Bilirubin calibration sample (with bilirubin blank)

Calibrating the Direct Bilirubin method using the Total Bilirubin calibration sample and to assign a bilirubin blank, enter the following information at the Blank Reagent Settings window.

No	Blank CH item		KV	BLK item		STD item
	No	Test Name		No	Test Name	No	Test Name
1	23	T-Bil	1	25	Bil-B
2	24	D-Bil	1	25	Bil-B	23	T-Bil
3	25	Bil-B				23	T-Bil

NOTE

The T-Bil and D-Bil test numbers might be different on your system.

Explanation of the Entries

Line 1 Bil-B is assigned as the blank channel to T-Bil.

Line 2 Bil-B is assigned as the blank channel to D-Bil, and the T-Bil calibration sample absorbance is assigned for calibration of the D-Bil channel.

Line 3 The T-Bil calibration sample absorbance is assigned for calibration of the Bil-B channel.

Operational Notes

Do not run a calibration sample for D-Bil even though its Calc. Mthd field is set to STD at the Analytical Parameters (Chemistry) window.
The sample results are calculated as follows:

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NOTE

The KV for D-Bil is not used in the calculations.