Multi-Standards Set window

Use this window to set standard values for a method when it uses multipoint calibration. At this window, you set standard solution to dilute at different rates, so the calibration curve can be created automatically (using the various dilutions).

Using the window

1. At the Analytical Parameters (Chemistry) window, verify that the Calc.mthd box is set to MSTD, then select Multipoint Cal Setting.

2. At the Multi-Standard Set window, select a formula, axis conversion, and number of points.

3. For each point used in the calibration (BLK numbers 1-5), enter the following:

·   Factor value (FV):  Factor value at a given point in the multipoint calibration.

·   Dilution method: Dilution method sets the manner in which this method handles dilution at each point in the multipoint calibration. Select the arrow, then select one of the following options:

·   Standard sample volume (Dil.smp vol):  The volume of calibrator (standard sample) used at each point in this method’s multipoint calibration. Possible values: 0–30 mL.

·   Diluent volume: The volume of diluent used with the calibrator in this method (for a point in the multipoint calibration). Possible values: 0–150 mL.

·   Diluent position:  The CTT position of the diluent used (on the calibrator) for a point in the multipoint calibration. Possible values: 1–61. If 0 is entered, standard diluent is used.

·   Standard value limits (Max Fit Deviation): The upper and lower limits (at each point in a multipoint calibration) for concentration values of calibrators. The limits set a range of normal values. If the measurement falls outside the range, the appropriate mark (H or L) is reported. If you do not want to set a range for a given point, set the H value at 9.99999 and the L value at -9.9999.

·   Maximum replication deviation (Max. Rep Deviation): The maximum replication deviation is level specific. The limits set a range of normal values. If the measurement falls outside the range, the appropriate mark (H or L) is reported. If you do not want to set a range for a given point, set the H value at 9.99999 and the L value at -9.9999.

·   Multi standard solution:  When the multipoint calibration uses a different standard solution for each point, perform the following actions:

4. Set the same values for dilution method, sample volume, diluent volume, and diluent position as found in the Analytical conditions section (serum samples) for this method.

5. In the FV boxes, set the displayed concentration of each solution.

·   Single standard solution: When the multipoint calibration uses the same standard solution for all points, perform the following actions:

6. Set the values for dilution method, sample volume, diluent volume, and diluent position for a dilution series by changing the dilution factor.

7. In the FV boxes, set the values according to the formula set in the Formula box.

·   Curve Type:  Sets how the system assigns the u, U, d, and D flags for absorbance flagging

·   Maximum Rep Deviat:  Maximum allowed deviation of calibrator replicate

Note

There is a Maximum Rep Deviation for each Factor Value (FV).

·   Min. No Rep:  Minimum number of calibrator replicates

·   Min. Abs Delta Lhi&endash;Llow:  Minimum allowable absorbance differences between the highest calibrator level (Lhi) and the lowest (Llow)

·   Max. RMS of Fit:  Maximum allowable root mean square calculation

8. Select OK to save the settings and return to the Analytical Parameters (Chemistry) window.