ISE Maintenance - As required

Washing all the lines

To wash all lines

1. At the Menu Panel, click Maint., then click ISE Operation.

2. Remove the two screws (1) that secure the shelf on which the buffer-solution bottle (2) is placed, then being careful not to damage any of the tubing, pull out the shelf. Replace the buffer solution with another buffer bottle containing 500 mL of deionized water.

3. Replace the Na, K, and Cl electrodes with a dummy electrode.

4. At the ISE Operation window, in the Bufferprime area, enter 50 into the Times field.

5. Select Execute.

6. Select Yes when prompted to execute buffer prime.

Washing the lines

1. Remove the buffer bottle with the deionized water and replace it with a bottle filled with a solution of 475 mL of deionized water and 25 mL probe wash 3 solution.

2. At the ISE Operation window, in the Bufferprime area, enter 50 into the Times field.

3. Select Execute.

4. Select Yes when prompted to execute buffer prime.

Rinsing the lines

1. Replace the probe wash 3 solution bottle with a bottle of deionized water.

2. Enter 50 again in the Times field of the Bufferprime area, then select Execute.

3. Remove the dummy electrode.

4. Reinstall the Na, K, and Cl electrodes  

5. In the Initialize area,  select Execute.

6. Before reinstalling the buffer-solution bottle, thoroughly rinse the buffer bottle cap, float switch, and tube with deionized water and dry completely.

7. Install a new bottle of buffer solution.

8. At the ISE Operation window, in the Bufferprime area, enter 15 into the Times field. Select Execute, then select Yes to prime the line with buffer.

9. When the priming is finished  verify that the electrodes are not leaking.

10. At the ISE Operation window, select Exit, then select Yes.

11. Run ten pooled serum samples, or do an ISE CV check.

12. Perform calibration and run controls.