ISE Maintenance - As required (refer to the schedule)

Washing the electrodes lines

Materials required

Biohazard

Time: 10 minutes

Analyzer mode: Manual operation

Wear personal protective equipment.
Use universal precautions.

Before you begin the task, do the following:

  1. At the Menu Panel, select Maint., then select ISE Operation.

To wash the electrode lines

  1. Remove the Na, K,  Cl, and Ref electrodes and install the dummy electrode in their place.

 WARNING

ISE detergent is a sodium hypochlorite solution. When handling bleach wear protective clothing, gloves, and safety glasses. It is harmful if swallowed and may cause eye or skin irritation. In case of skin or eye contact, flush with large amounts of water.

  1. Remove the cap of the dummy electrode and put about 5 ml of ISE detergent solution into the dummy electrode.

 CAUTION

Be sure to tighten the cap on the dummy electrode. If the cap is loose or defective, the ISE detergent solution may leak into the module and cause damage.

  1. Replace and tighten the cap.

  2. At the ISE Operation window, select Execute (STEP-1) to the right of  ISE line wash.  

The message "Line Wash 1 Running" and the approximate amount of time remaining appears in the display area. You cannot stop this operation. If you must stop, press the SYSTEM STOP button on the analyzer display panel.

  1. After step 1 of the wash is completed, replace the dummy electrode with the original Na, K, Cl, and Ref electrodes.  

  2. At the ISE Operation window, select Execute (STEP-2) to the right of  ISE line wash.

The wash starts and buffer prime is performed 10 times. The message "Line Wash 2 Running" and the approximate amount of time remaining appears in the display area.

  1. Verify that there are no leaks or bubbles and that the buffer is going to the waste during the priming cycle.

  2. At the ISE Operation window, next to the Initialize button, select Execute.

  3. Perform calibration and run controls.