Checking lamp energy

After cleaning or replacing cuvettes or replacing the lamp, check the lamp energy.

Materials required

Biohazard

Time: 15 minutes

Analyzer mode: READY

Wear personal protective equipment.
Use universal precautions.

To check lamp energy

IMPORTANT

Do not touch or turn the reaction tray at any time during the lamp energy check procedure. The reaction tray should turn freely. If the reaction tray is shifted, it could result in an erroneous lamp energy reading.

  1. From the Menu Panel, select Maint., then select Lamp Energy Monitor.

The Lamp Energy Monitor window appears.

Help with the Lamp Energy Monitor window

  1. Ensure that the bottle at position #50 in Reagent Tray 1 (RTT1) contains clean deionized water.

  2. Select Check energy.

The Lamp Energy Monitor dialog box appears.

  1. Type 50 in the RTT1 bottle posi. field and select 3: 70 ml for the Container field. Select Meas. Start.

·    The reagent probe aspirates deionized water from RTT1 and dispenses it into reaction cuvette #1.

·   The reaction disk rotates until cuvette #1 is in the detection position.

·   The Operation window displays Lumi.Check and then WAIT.
Perform steps 5-9 in the WAIT state.

  1. On the Lamp Energy Monitor window, in the Luminous Energy check area

  1. Type 1000 in the Meas. times field.

  2. Type 100 in the Meas. cycle field.

  3. Select AD.

  4. Select Auto.

  1. Select Meas. Energy. The message "Execute the lamp energy check?" appears.

  2. Select OK.

  3. On the Lamp Energy Monitor window, select Collect Data.

  4. Calculate the scatter plot.

  1. Add 50 to the 340-nm AD count and enter the sum in the top field to the left of the graph. Press Enter.

  2. Subtract 50 from the 340-nm AD count and enter the difference in the bottom field to the left of the graph. Press Enter.

The lamp energy appears as a scatter plot.

·    If the points are mostly within ±40 of the center, the lamp is normal.

·    Otherwise, replace the lamp.

  1. Only if you replaced the lamp or a cuvette segment, select Regist. Data, and then select OK in the Registration window. If not, proceed to step 11.

Note

The system uses the data from the lamp energy data registration as the comparison standard for the next calculation of the attenuation ratio.

  1. Exit the Lamp Energy Monitor window, then select Initialize to switch the system from the WAIT state to the READY state.

 

Run cell blank measurement.