Cleaning and replenishing the dilution bottle

Materials required  

Biohazard

Time:10 minutes

Analyzer mode: READY

Wear personal protective equipment.
Use universal precautions.

Cleaning the dilution bottle can be performed at time of bottle refill, but must be performed at least once a month.

 

 

 

1    ISE buffer bottle 

2     RRV (Reaction) bath oil bottle

3     Isotonic saline diluent bottle

4     Cuvette detergent bottle

5    Cell conditioner bottle

To clean the dilution bottle

  1. Lift the cover from the saline diluent bottle (3), and remove the bottle.

 Caution

To avoid mixing up the fluid bottles, make a note of the bottle position on the shelf.

  1. Empty the remaining contents of the bottle.

  2. Rinse the bottle with deionized water and drain well.

  3. Refill the bottle with 0.9% saline diluent.

  4. Replace the bottle in the same position on the shelf in the cabinet.

  5. Replace the cover of the diluent bottle.

NOTE

Make sure that the Teflon tube and filter holder are located at the bottom of the dilution bottle.

  1. Prime the fluid lines.

NOTE

If you are cleaning the detergent or cell conditioner bottles at this time, you can prime all the fluid lines at once.

  1. At the Operation Panel, select the Prime button.

Using the Prime Set window

  1. At the PRIME Set dialog box, select Prime 2 and enter 10 or more for the number of times in all fields.

  2. Select Execute.