Use the View Calibration Curve window to review the detailed calibration curve data for a method, to restore a calibration, to monitor calibration trends, to obtain a summary of all calibration information and to view RBL and calibration check information.
At the Menu Panel, select Calibration, then select View Calibration Curve.
Perform any of the following tasks on this window:
Close the window.
Notes
· The system stores up to 2 separate calibration curves for each method, based on the reagent lot numbers. If calibration is performed on a new lot of R1, R2, or both, the software recognizes it as a new reagent lot pairing and stores a second calibration curve for that method.
· The reagent container for which you want to view the calibration curve, must be loaded in the reagent tray, because each calibration curve is associated with a reagent lot number.
· Calibrations with a Fail status are also saved for review. The system stores only the most recent failed calibration for a method, regardless of reagent lot numbers.
At the View Calibration Curve window, in the Test name list, select the applicable method.
Select the curve to display:
· Curve 1 is an active curve resulting from a successful calibration for the selected method.
· Curve 2 is an active curve for the same method, but for a different reagent lot than Curve 1.
· Failed displays the data from the most recent calibration with a status of Fail for the selected method.
The calibration curve, pass/fail status, and associated information displays.
The system automatically scales the calibration curve.
To apply different scaling values, do the following:
Select Scale change.
Enter the absorbance range you want for each axis.
Select OK.
NOTE
The entries in the Calib.curve info. area determine the calibration type. Do not change these entries.
In the Statistical data area, review the FV, MEAN, R, N for the blank and standard samples, and User.
For multipoint calibration methods, review the curve coefficients (A through E).
If you performed a simplified calibration for a multi-point calibration method, the Blank, STD, SD, Sr, and r values display at the lower left of the window. Additionally, both the original and corrected calibration curves are plotted.
Failed calibration data can be replaced by restoring or activating a previously successful calibration for the same reagent lot pair. You must run control samples and establish that the QC results meet the laboratory’s criteria for acceptability. This feature enables the system to run the method using previous calibration data.
This function requires a supervisor level password.
At the View Calibration Curve window, select the appropriate method from the Test name list.
Select Curve 1 or Curve 2, to display the curve for the appropriate reagent lot number that you want to restore.
Select Restore Curve.
When you restore a calibration, the date and time of the restored calibration is recorded as today's date and not the date of the original calibration. Also the calibration interval defaults to 1.
The Calibration Curve List window displays the date and time of each previously successful calibration for the selected reagent lot pair.
Select the date and time, to identify the calibration to restore.
Note
The status of the calibration changes from passed to restored. A flag is not posted to test results obtained using this restored curve.
At the View Calibration Curve window, select Cal Curve History.
At the Cal Curve History window, in the Test Name list, select the method to display.
Notes
· The calibration curve displayed in the View Calibration Curve window is not automatically selected.
· The blank trace, the standard trace, and their associated statistical data display.
· The system automatically scales the calibration curve.
To apply different scaling values, do the following:
Select Scale change.
Enter the absorbance range you want for each axis.
Select OK.
To print the calibration trace data:
Select Print.
At the Print window, select one of the following options:
· Current Test Selection – prints the data for the previously selected method.
· Selection – allows you to select one or more methods from the displayed list to print or Select All to print data for every test in the list.
To print the trace diagram with the data, select Print Graph.
Select Print.
At the View Calibration Curve window, select Calibration Summary.
The following calibration information displays for each method: No., Test Name, Classif, FV, Unit, BLANK, STD, E2-BLANK, F and User.
To print out the summary, select Print.
To change the calibration data, use the text boxes in the Edit.tests area.
IMPORTANT
To avoid generating erroneous results, exercise caution if you must edit the calibration data. Be sure to carefully evaluate control results before running patient samples.
To save any changes and exit, select Return.
To exit without saving changes, select Cancel.
At the View Calibration Curve window, select RBL Check.
The RBL Check Results window opens and displays the following information:
· Reagent Blank (RBL)
· Value of RBL
· Precision
· RBL Tracking
To close the window, select Return.
At the View Calibration Curve window, select Cal Check.
The Calibration Check Results window opens and displays the following information:
For Single-Point Calibrations:
· Reagent and Calibrator Lot Information
· Value of Calibration
· Precision
· Calibration Tracking
For Multi-Point Calibrations:
· Reagent and Calibrator Lot Information
· Precision of Replicates
· Absorbance Changes
· RMS Data
· Curve Parameters
· Calibration Fit
To close the window, select Return.