Using simplified calibration

Overview

Simplified calibration enables the system to adjust a multi-point calibration curve by running the blank and the highest-concentration calibrator.

To use this feature, first run a valid multi-point calibration using all the calibration standards defined on the STT for the method. Subsequently, perform a simplified calibration by assaying the blank and highest-concentration calibrator from the CTT. Edit the new absorbance data in the View Calibration window to create a new calibration curve.

Procedure

  1. In the BLK posi. field of the Calibration Setup window, type the CTT position of the blank calibration standard.

  2. In the STD posi. field, type the CTT position of the highest-concentration calibrator.

  3. Select CTT Set to open the CTT Settings window.

  4. In Cup/Tube Type list, select the blank container type.

  5. In Times field, type the number of aspirations for the blank.

  6. Repeat steps 4 and 5 for the highest-concentration calibrator.

  7. Save the changes.

  8. In the Item Select area of the Test Select window, select Ordinary Calibration.

  9. In the Test table area, select the check box of the method.

  10. Save the changes.

  11. At the Sample Select window, select diluent and highest-concentration calibrator CTT positions.

  12. Save the changes.

  13. Run calibration:

  1. At the Start Conditions window, select one-pnt,smp analyze.

  2. Use the temporary item select and temporary sample selections if necessary.

  3. Select Start.

note

To run calibration using only the blank, deselect the other calibrator from the temporary sample selections.

  1. When the processing is completed, open the View Calibration window.

  2. Select Test select.

  3. In the Test Select list, select the applicable method, then select OK.

  4. In the last two fields of the Statistical data area, locate and record the new BLANK and STD absorbance data in the Mean column that represent the results of the simplified calibration.

  5. Select Calib.monitor. to open the Calibration Monitor window.

  6. Select the method.

  7. In the Edit.items area in the upper right-hand corner of the window, enter the new absorbance data for FV and BLANK.

You must manually enter the values for FV and Blank.

  1. Select Return.

  2. Select Reapproxim.

  3. When prompted for "Re-approximation processing?", select Yes.

  4. When prompted for "Carry out simplified re-approximation?", select Yes.

The calibration graph updates to show the calibration and corrected curves.

  1. Save the new calibration data.