Checking Reagents

Checking the availability of the reagents

  1. Visually check the system reagents.

  2. Perform a prime after replacing any system ancillary reagents.

  1. At the Operation Panel, select Prime.

  2. Select PRIME 2, then select Execute.

  1. Visually check the ISE reagents.

  2. Perform a prime after replacing the ISE Buffer.

  1. At the Menu Panel, select Maint., then select ISE Operation.

  2. In the Times box of the Bufferprime area, type the number of prime cycles desired (20 is recommended).

  3. Select Execute.

  1. Visually check the controls and calibrators on the calibrator/control tray.

  2. Visually check the wash solutions.

  3. Visually check the lamp coolant level.

  4. Check the method reagents in the reagent trays.

  1. At the Menu Panel, select Reagent, then select Reagent Inventory.

  2. At the Reagent Inventory window, determine if any reagents need replenishing.

  3. Replace any expired reagents.

 caution

You must perform a barcode scan following any changes you make to the reagent containers on the RTT1 and RTT2 trays. Failure to scan after moving, adding, or deleting reagents can cause erroneous results.

If you accidentally switch barcoded reagents (R1 reagent on RTT-2 and R2 reagent on RTT1), and perform a reagent barcode scan, an error message displays.

·   Replace barcoded reagent containers

·   Replace nonbarcoded reagent containers

note

If you replace only 1 of the reagents of a 2-reagent method and the replaced reagent has a new lot number, after the barcode scan, the system alerts you that a reagent pair does not exist. You must calibrate the new set of reagents before you continue to run samples.

  1. After replacing the reagents, do the following:

(1)   At the Menu Panel, select Reagent, then select Reagent Inventory.

(2)   Execute a Barcode Scan at the Reagent Inventory window.

  1. Evaluate calibration status.